Wednesday, June 10, 2009
Carbon nanotube-based transducers for immunoassays
The attachment of mouse immunoglobulin G (IgG) and anti-mouse IgG antibodies onto carbon nanotubes (CNTs), using either non-covalent or covalent means was investigated. The resultant CNTs were characterised using a variety of techniques including enzyme-linked and fluorescence-linked immunoassays, UV–visible-NIR and Raman spectroscopy, transmission electron microscopy and cyclic voltammetry.
TEM images of the adsorbed antibody on the CNTs show that the covalent modification approach was successful, whereas the non-covalent approach resulted in no electrochemically detectable labelled antibody. Direct electrical communication between CNTs covalently linked to peroxidase-labelled antibodies was observed during cyclic voltammetry, which suggests applications in developing carbon-nanotube-based immunosensors.
(Carol Lynam, Niamh Gilmartin, Andrew I. Minett, Richard O’Kennedy, and Gordon Wallace, Carbon, Article in Press, 2009, doi:10.1016/j.carbon.2009.04.017)
TEM images of the adsorbed antibody on the CNTs show that the covalent modification approach was successful, whereas the non-covalent approach resulted in no electrochemically detectable labelled antibody. Direct electrical communication between CNTs covalently linked to peroxidase-labelled antibodies was observed during cyclic voltammetry, which suggests applications in developing carbon-nanotube-based immunosensors.
(Carol Lynam, Niamh Gilmartin, Andrew I. Minett, Richard O’Kennedy, and Gordon Wallace, Carbon, Article in Press, 2009, doi:10.1016/j.carbon.2009.04.017)
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